Abstract
Background aims
Bone marrow-derived hematopoietic stem cell transplantation/hematopoietic progenitor
cell transplantation (HSCT/HPCT) is widely used and one of the most useful treatments
in clinical practice. However, the homing rate of hematopoietic stem cells/hematopoietic
progenitor cells (HSCs/HPCs) by routine cell transfusion is quite low, influencing
hematopoietic reconstitution after HSCT/HPCT.
Methods
The authors developed a micro-living motor (MLM) strategy to increase the number of
magnetically empowered bone marrow cells (ME-BMCs) homing to the bone marrow of recipient
mice.
Results
In the in vitro study, migration and retention of ME-BMCs were greatly improved in comparison with
non-magnetized bone marrow cells, and the biological characteristics of ME-BMCs were
well maintained. Differentially expressed gene analysis indicated that ME-BMCs might
function through gene regulation. In the in vivo study, faster hematopoietic reconstitution was observed in ME-BMC mice, which demonstrated
a better survival rate and milder symptoms of acute graft-versus-host disease after
transplantation of allogeneic ME-BMCs.
Conclusions
This study demonstrated that ME-BMCs serving as MLMs facilitated the homing of HSCs/HPCs
and eventually contributed to earlier hematopoietic reconstitution in recipients.
These data might provide useful information for other kinds of cell therapies.
Graphical abstract
Graphical Abstract
Key Words
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Article info
Publication history
Published online: December 08, 2022
Accepted:
November 9,
2022
Received:
April 10,
2022
Identification
Copyright
© 2022 Published by Elsevier Inc. on behalf of International Society for Cell & Gene Therapy.
