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Magnetically empowered bone marrow cells as a micro-living motor can improve early hematopoietic reconstitution

  • Author Footnotes
    # These authors contributed equally to this work.
    Qiusui Mai
    Footnotes
    # These authors contributed equally to this work.
    Affiliations
    Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China

    Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
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  • Author Footnotes
    # These authors contributed equally to this work.
    Zhengyuan Wang
    Footnotes
    # These authors contributed equally to this work.
    Affiliations
    Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China
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  • Author Footnotes
    # These authors contributed equally to this work.
    Quanfeng Chen
    Footnotes
    # These authors contributed equally to this work.
    Affiliations
    Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China
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  • Jialu Zhang
    Affiliations
    Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China
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  • Dingyi Zhang
    Affiliations
    Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China
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  • Chengyao Li
    Correspondence
    Correspondence: Chengyao Li, PhD, Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou Avenue North, No. 1838, Guangzhou 510515, China.
    Affiliations
    Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
    Search for articles by this author
  • Qianli Jiang
    Correspondence
    Correspondence: Qianli Jiang, MD, PhD, Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou Avenue North, No. 1838, Guangzhou 510515, China.
    Affiliations
    Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China
    Search for articles by this author
  • Author Footnotes
    # These authors contributed equally to this work.
Published:December 08, 2022DOI:https://doi.org/10.1016/j.jcyt.2022.11.002

      Abstract

      Background aims

      Bone marrow-derived hematopoietic stem cell transplantation/hematopoietic progenitor cell transplantation (HSCT/HPCT) is widely used and one of the most useful treatments in clinical practice. However, the homing rate of hematopoietic stem cells/hematopoietic progenitor cells (HSCs/HPCs) by routine cell transfusion is quite low, influencing hematopoietic reconstitution after HSCT/HPCT.

      Methods

      The authors developed a micro-living motor (MLM) strategy to increase the number of magnetically empowered bone marrow cells (ME-BMCs) homing to the bone marrow of recipient mice.

      Results

      In the in vitro study, migration and retention of ME-BMCs were greatly improved in comparison with non-magnetized bone marrow cells, and the biological characteristics of ME-BMCs were well maintained. Differentially expressed gene analysis indicated that ME-BMCs might function through gene regulation. In the in vivo study, faster hematopoietic reconstitution was observed in ME-BMC mice, which demonstrated a better survival rate and milder symptoms of acute graft-versus-host disease after transplantation of allogeneic ME-BMCs.

      Conclusions

      This study demonstrated that ME-BMCs serving as MLMs facilitated the homing of HSCs/HPCs and eventually contributed to earlier hematopoietic reconstitution in recipients. These data might provide useful information for other kinds of cell therapies.

      Graphical abstract

      Key Words

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