Abstract
Mesenchymal stromal cells (MSCs) have shown potential therapeutic benefits for a range
of medical disorders and continue to be a focus of intense scientific investigation.
Transplantation of MSCs into injured tissue can improve wound healing, tissue regeneration
and functional recovery. However, implanted cells rapidly lose their viability or
fail to integrate into host tissue. Hydrogel-seeded bone marrow (BM)-MSCs offer improved
viability in response to mechanical forces caused by syringe needles, cell density
and dimethylsulfoxide (DMSO) concentration, which in turn, will help to clarify which
factors are important for enhancing biomaterial-induced cell transplantation efficiency
and provide much needed guidance for clinical trials. In this study, under the control
of cell density (<2 × 107 cells/mL) and final DMSO concentration (<0.5%), hydrogel-induced BM-MSC viability
remained >82% following syringe needle passage by 25- or 27-gauge needles, providing
improved cell therapeutic approaches for regenerative medicine.
Key Words
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Article info
Publication history
Published online: October 04, 2017
Accepted:
August 23,
2017
Received:
April 27,
2017
Identification
Copyright
© 2017 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
