Cryopreservation is widely used to generate large stocks or cell banks that can be stored for short and long periods ready to be recovered in a functionally potent state and used as required in a cell therapy application. Animal based reagents are usually employed in cryopreservation media as a source of nutrients and other perceived benefits during the post-thaw period. Current evidence suggest that the animal serum proteins used in cryopreservation media are difficult to remove during post-thaw washing, and any residual proteins in cells may trigger adverse reactions in the patients who receive cell infusions or transplants. Therefore, demand is increasing for cryopreservation media to be devoid of any animal derived components, especially when cells are intended for cell therapy application. Human platelet lysate (HPL) provides an animal component free option without the risks and regulatory challenges associated with animal derived components. In this study we investigated HPL for its ability to recover functionally potent cells following thawing and subsequent culture in cryopreservation of human derived mesenchymal stem cells (MSC). The results indicated HPL can improve cell recovery and functionality following thawing and provides an opportunity to develop animal protein free regulatory compliant cryopreservation media for cell therapy applications.
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© 2015 Published by Elsevier Inc.