Advertisement
Letter to the editor| Volume 16, ISSUE 12, P1771-1773, December 2014

Cryopreservation can be used as an anti-aging strategy

  • Mahmood S. Choudhery
    Correspondence
    Correspondence: Mahmood S. Choudhery, PhD, Tissue Engineering and Regenerative Medicine Laboratory, Center of Research in Biomedical Sciences, King Edward Medical University/Mayo Hospital, Lahore, Pakistan.
    Affiliations
    Tissue Engineering and Regenerative Medicine Laboratory, Advance Center of Research in Biomedical Sciences/King Edward Medical University/Mayo Hospital, Lahore, Pakistan

    Department of Immunobiology, College of Medicine, the University of Arizona, Tucson, Arizona, USA
    Search for articles by this author
  • David T. Harris
    Affiliations
    Department of Immunobiology, College of Medicine, the University of Arizona, Tucson, Arizona, USA
    Search for articles by this author
Published:October 07, 2014DOI:https://doi.org/10.1016/j.jcyt.2014.08.012
      Advanced age is a major risk factor for various diseases and disorders (such as cardiovascular and neurodegenerative disorders), highlighted by diminished repair and differentiation potential of stem cells limiting their potency for treatment. Recent achievements in the field of tissue engineering and regenerative medicine are very promising. The preclinical data that used stem cells isolated from adult tissues such as adipose, bone marrow and neonatal sources (umbilical cord blood and cord tissue) are very auspicious. As a result, several hundred preclinical and clinical trials have been registered with the US Food and Drug Administration that are at various stages of completion (see www.clinicaltrials.gov). Although these technological advancements have opened new possibilities for the treatment of various diseases, there are a number of issues still to resolve. For example, neonatal sources are rich in stem cells but can be obtained only once in a lifetime. Autologous stem cell therapy appears the most practical option for cell-based therapy; however, the regenerative ability of stem and progenitor cells is severely compromised with increasing donor age. Similarly, autologous stem cells such as those obtained from adipose tissue or bone marrow can be used for cell therapy or for tissue engineering only if available in sufficient number. Although expansion may provide increased cell numbers and higher purity, it leads to reduced cell function (limits proliferative and differentiation capabilities) as the result of cell aging, increased pecuniary cost and increased risk of contamination with microorganisms. Therefore, preservation of stem and progenitor cells (from adipose tissue and bone marrow) at a young age (or at the time of delivery in the case of cord blood and cord tissue) while maintaining their youthful biological activities and potential could provide an ideal situation for future regenerative medicine applications. In addition, cryopreservation of cells and tissues could avoid repeated in vitro culture expansion because the cells and tissues obtained during different procedures at various time points could be cryopreserved and pooled at the time of care when specific medical conditions need transplantations.

      Key Words

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Cytotherapy
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Janzen V.
        • Forkert R.
        • Fleming H.E.
        • Saito Y.
        • Waring M.T.
        • Dombkowski D.M.
        • et al.
        Stem-cell ageing modified by the cyclin dependent kinase inhibitor p16INK4a.
        Nature. 2006; 443: 421-426
        • Stolzing A.
        • Sethe S.
        • Scutt A.M.
        Stressed stem cells: temperature response in aged mesenchymal stem cells.
        Stem Cells Dev. 2006; 15: 478-487
        • Rattan S.I.
        • Singh R.
        Progress and prospects: gene therapy in aging.
        Gene Ther. 2009; 16: 3-9
        • Choudhery M.S.
        • Badowski M.
        • Muise A.
        • Harris D.T.
        Utility of cryopreserved umbilical cord tissue for regenerative medicine.
        Curr Stem Cell Res Ther. 2013; 8: 370-380
        • Choudhery M.S.
        • Badowski M.
        • Muise A.
        • Pierce J.
        • David D.T.
        Cryopreservation of whole adipose tissue for future use in regenerative medicine.
        J Surgical Res. 2014; 187: 24-35