Abstract
Background aims
Recent advances in stem cell research have raised the possibility of stem cells repairing
or replacing retinal photoreceptor cells that are either dysfunctional or lost in
many retinal diseases. Various types of stem cells have been used to replace retinal
photoreceptor cells. Recently, peripheral blood stem cells, a small proportion of
pluripotent stem cells, have been reported to mainly exist in the peripheral blood
mononuclear cells (PBMCs).
Methods
In this study, the effects of pre-induced adult human PBMCs (hPBMCs) on the degenerative
retinas of rd1 mice were investigated. Freshly isolated adult hPBMCs were pre-induced with the use
of the conditioned medium of rat retinas for 4 days and were then labeled with chloromethyl-benzamidodialkylcarbocyanine
(CM-DiI) and then transplanted into the subretinal space of the right eye of rd1 mice through a trans-scleral approach. The right eyes were collected 30 days after
transplantation. The survival and migration of the transplanted cells in host retinas
were investigated by whole-mount retinas, retinal frozen sections and immunofluorescent
staining.
Results
After subretinal transplantation, pre-induced hPBMCs were able to survive and widely
migrate into the retinas of rd1 mice. A few CM-DiI–labeled cells migrated into the inner nuclear layer and the retinal ganglion
cell layer. Some transplanted cells in the subretinal space of rd1 host mice expressed the human photoreceptor–specific marker rhodopsin.
Conclusions
This study suggests that pre-induced hPBMCs may be a potential cell source of cell
replacement therapy for retinal degenerative diseases.
Key Words
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Article info
Publication history
Accepted:
May 22,
2013
Received:
December 4,
2012
Identification
Copyright
© 2013 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
