Cell expansion for clinical applications is mainly performed in the presence of fetal
calf serum (FCS). As FCS is known to cause disease transmission and incorporation
of xenogenic proteins during cultivation, alternative supplements for cell culture
media are desired. In recent years, human serum (huS) and human platelet lysate (hPL)
turned out to be as potential or even better when used instead of FCS. We established
production processes for huS and two different types of hPL in the last years. Now
their efficacy in application as cell culture supplement should be determined.
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© 2013 Published by Elsevier Inc.