Mapping and modelling ex vivo cell culture processes directly from cell tracking data

      Cost-effect culture methods for large-scale cell expansion and directed differentiation are central considerations for developing cell therapy products. However, it is not possible to directly quantify the rates of cellular events that govern productivity from bulk culture observations, namely mitosis, apoptosis and differentiation. The goal of this research is to develop live cell imaging tools to gain a deeper understanding of the cell expansion and differentiation process. Initiating cells and their progeny are continuously tracked by live cell imaging to determine how ex vivo manipulations influence probabilistic single cell fates. Our initial focus was to characterise granulocyte/macrophage progenitor and cardiac mesenchymal-like lineage expansion, however a similar approach can be applied to virtually any cell expansion product.
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