Abstract
Background aims
Pancreatic cancer, sometimes called a ‘silent killer’, is one of the most aggressive
human malignancies, with a very poor prognosis. It is the fourth leading cause of
cancer-related morbidity and mortality in the USA.
Methods
A mouse peritoneal model was used to test the ability of unengineered rat umbilical
cord matrix-derived stem cells (UCMSC) to control growth of pancreatic cancer. In vivo results were supported by various in vitro assays, such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT),
direct cell count, [3H]thymidine uptake and soft agar colony assays.
Results
Co-culture of rat UCMSC with PAN02 murine pancreatic carcinoma cells (UCMSC:PAN02,
1:6 and 1:3) caused G0/G1 arrest and significantly attenuated the proliferation of
PAN02 tumor cells, as monitored by MTT assay, direct cell counts and [3H]thymidine uptake assay. Rat UCMSC also significantly reduced PAN02 colony size and
number, as measured by soft agar colony assay. The in vivo mouse studies showed that rat UCMSC treatment significantly decreased the peritoneal
PAN02 tumor burden 3 weeks after tumor transplantation and increased mouse survival
time. Histologic study revealed that intraperitoneally administered rat UCMSC survived
for at least 3 weeks, and the majority were found near or inside the tumor.
Conclusions
These results indicate that naive rat UCMSC alone remarkably attenuate the growth
of pancreatic carcinoma cells in vitro and in a mouse peritoneal model. This implies that UCMSC could be a potential tool
for targeted cytotherapy for pancreatic cancer.
Key Words
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Article info
Publication history
Accepted:
December 3,
2009
Received:
July 23,
2009
Identification
Copyright
© 2010 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
