Advertisement
Rapid Communication| Volume 12, ISSUE 3, P332-337, 2010

Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes

DOI:https://doi.org/10.3109/14653240903548202
Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes
Previous ArticleDirectional differentiation of chicken spermatogonial stem cells in vitro
Next ArticleGood manufacturing practice-grade production of unrestricted somatic stem cell from fresh cord blood

      Abstract

      Background aims

      The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue.

      Methods

      Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture.

      Results

      After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fibers), immunofluorescence detection of cardiac-specific markers (connexin-43, sarcomeric α-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription–polymerase chain reaction (cardiac myosin light chain 1, α-cardiac actin, cardiac troponin T and cardiac β-myosin).

      Conclusions

      We have demonstrated for the first time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease.

      Key Words

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Cytotherapy
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Laflamme M.A.
        • Murry C.E.
        Regenerating the heart.
        Nat Biotechnol. 2005; 23: 845-856
        View in Article
        • Urbanek K.
        • Cesselli D.
        • Rota M.
        • Nascimbene A.
        • de Angelis A.
        • Hosoda T.
        • et al.
        Stem cell niches in the adult mouse heart.
        Proc Natl Acad Sci USA. 2006; 103: 9226-9231
        View in Article
        • Guan K.
        • Hasenfuss G.
        Do stem cells in the heart truly differentiate into cardiomyocytes?.
        J Mol Cell Cardiol. 2007; 43: 377-387
        View in Article
        • Romanov Y.A.
        • Darevskaya A.N.
        • Merzlikina N.V.
        • Buravkova L.B.
        Mesenchymal stem cells from human bone marrow and adipose tissue: isolation, characterization, and differentiation potentialities.
        Bull Exp Biol Med. 2005; 140: 138-143
        View in Article
        • Håkelien A.M.
        • Landsverk H.B.
        • Robl J.M.
        • Skålhegg B.S.
        • Collas P.
        Reprogramming fibroblasts to express T-cell functions using cell extracts.
        Nat. Biotechnol. 2002; 20: 460-466
        View in Article
        • Jin X.B.
        • Sun Y.S.
        • Zhang K.
        • Wang J.
        • Ju X.D.
        • Lou S.Q.
        Neocartilage formation from predifferentiated human adipose derived stem cells in vivo.
        Acta Pharmacol Sin. 2007; 28: 663-671
        View in Article
        • Gaustad K.G.
        • Boquest A.C.
        • Anderson B.E.
        • Gerdes A.M.
        • Collas P.
        Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes.
        Biochem Biophys Res Commun. 2004; 314: 420-427
        View in Article
        • Gimble J.M.
        • Katz A.J.
        • Bunnell B.A.
        Adipose-derived stem cells for regenerative medicine.
        Circ Res. 2007; 100: 1249-1260
        View in Article
        • Makino S.
        • Fukuda K.
        • Miyoshi S.
        • Konishi F.
        • Kodama H.
        • Pan J.
        • et al.
        Cardiomyocytes can be generated from marrow stromal cells in vitro.
        J Clin Invest. 1999; 103: 697-705
        View in Article
        • Moore J.C.
        • Tsang S.Y.
        • Rushing S.N.
        • Lin D.
        • Tse H.F.
        • Chan C.W.
        • et al.
        Functional consequences of overexpressing the gap junction Cx43 in the cardiogenic potential of pluripotent human embryonic stem cells.
        Biochem Biophys Res Commun. 2008; 377: 46-51
        View in Article
        • Höllrigl A.
        • Hofner M.
        • Stary M.
        • Weitzer G.
        Differentiation of cardiomyocytes requires functional serine residues within the amino-terminal domain of desmin.
        Differentiation. 2007; 75: 616-626
        View in Article
        • Bai X.
        • Pinkernell K.
        • Song Y.H.
        • Nabzdyk C.
        • Reiser J.
        • Alt E.
        Genetically selected stem cells from human adipose tissue express cardiac markers.
        Biochem Biophys Res Commun. 2007; 353: 665-671
        View in Article
        • Chen Z.
        • Huang W.
        • Dahme T.
        • Rottbauer W.
        • Ackerman M.J.
        • Xu X.
        Depletion of zebrafish essential and regulatory myosin light chains reduces cardiac function through distinct mechanisms.
        Cardiovasc Res. 2008; 79: 97-108
        View in Article
        • Rangappa S.
        • Entwistle J.W.
        • Wechsler A.S.
        • Kresh J.Y.
        Cardiomyocyte-mediated contact programs human mesenchymal stem cells to express cardiogenic phenotype.
        J Thorac Cardiovasc Surg. 2003; 126: 124-132
        View in Article
        • Miguel J.
        • Erices A.
        Mesenchymal stem cells and the treatment of cardiac disease.
        Exp Biol Med. 2006; 231: 39-49
        View in Article
        • Schimrosczyk K.
        • Song Y.H.
        • Vykoukal J.
        • Vykoukal D.
        • Bai X.
        • Krohn A.
        • et al.
        Liposome-mediated transfection with extract from neonatal rat cardiomyocytes induces transdifferentiation of human adipose-derived stem cells into cardiomyocytes.
        Scand J Clin Lab Invest. 2008; 68: 464-472
        View in Article
        • Koninckx R.
        • Hensen K.
        • Daniëls A.
        • Moreels M.
        • Lambrichts I.
        • Jongen H.
        • et al.
        Human bone marrow stem cells co-cultured with neonatal rat cardiomyocytes display limited cardiomyogenic plasticity.
        Cytotherapy. 2009; 11: 778-792
        View in Article
        • Takeuchi J.K.
        • Bruneau B.G.
        Directed transdifferentiation of mouse mesoderm to heart tissue by defined factors.
        Nature. 2009; 459: 654-655
        View in Article

      Article info

      Publication history

      Accepted: December 3, 2009
      Received: September 14, 2009

      Identification

      DOI: https://doi.org/10.3109/14653240903548202

      Copyright

      © 2010 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

      ScienceDirect

      Access this article on ScienceDirect
      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties. The content on this site is intended for healthcare professionals.


      RELX