Abstract
Background aims
Stem cells (SC) in different locations have individual characteristics. Important
questions to be answered include how these specialties are generated, what the mechanism
underlying their generation is, and what their biologic and clinical merits are. A
basic approach to answering these questions is to make comparisons between the differences
and similarities among the various SC types. They may focus on aspects of biologic
marker discovery, capacity of proliferation and differentiation, along with other
characteristics. The aim of this study was to characterize in detail the SC isolated
from pancreatic islet (PI) and compare their properties with bone marrow (BM)-derived
mesenchymal stromal cells (MSC) of the rat.
Methods
Immunophenotypic characteristics, proliferation capacities, telomerase activities,
pluripotent-related gene expressions, ultrastructure and the potential for multilineage
differentiation of PI SC and BM MSC were studied.
Results
We found that PI SC expressed markers of embryonic SC (Oct-4, Sox-2 and Rex-1) and
had a high proliferation capacity, proven also by high telomerase activities. Surprisingly,
markers belonging to differentiated cells were expressed by these cells in a constitutive
manner. PI SC ultrastructure showed more developed and metabolically active cells.
Conclusions
The immunocytochemical identification of both PI SC and BM MSC was demonstrated to
be typical MSC. Without stimulation of differentiation markers of adipogenic, chondrogenic,
neurogenic, myogenic and osteogenic cells in these SC, the expression of those markers
might explain their multilineage differentiation potential. We suggest that, by reason
of the respectively high telomerase activity in PI SC, they could be better candidates
than BM MSC for cell replacement therapy of type 1 diabetes.
Key Words
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Article info
Publication history
Accepted:
December 21,
2009
Received:
October 27,
2009
Identification
Copyright
© 2010 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
