Background
Primary cultures of isolated human adipose-derived adult stem (ADAS) cells are multipotent
and differentiate in vitro along the adipocyte, chondrocyte, neuronal, osteoblast,
and skeletal muscle pathways.
Methods
We examined the ADAS cell yield per unit volume of liposuction tissue, and their surface
protein phenotype by flow cytometry. Adipogenesis was assessed by Oil Red O staining
and ELISA analysis of leptin secretion.
Results
The donor population was 87.5% female (n = 18) with a mean age (± SD) of 44 ± 10 years and body mass index (BMI) of 24.9 + 2.7. The mean cell yield was 404 000 ± 206 000 cells per milliliter of lipoaspirate (n = 18). Linear regression analysis of the cells derived from the female donors demonstrated
a significant negative correlation between the number of cells obtained per milliliter
of lipoaspirate with the BMI but not the age of the donor.
The undifferentiated ADAS cells were homogeneously positive for the cell-surface markers
CD10, CD13, CD29, CD44, CD49e, CD59, CD90, and HLA-ABC, and homogeneously negative
for the cell surface markers CD11b, CD45, and HLA-DR. The absence of the panhema-topoietic
marker, CD45, indicates that the ADAS cells do not derive from circulating BM hematopoietic
stem cells. Adipocyte differentiation led to a 5.1-fold increase in Oil Red O staining,
and a 196-fold increase in leptin secretion levels. Culture of the cells in the presence
of antibiotic and fungizone did not alter the undifferentiated ADAS cell immunophenotype
based on flow cytometry, or their adipocyte differentiation based on leptin secretion.
Discussion
The ability to isolate a consistently homogeneous population of undifferentiated adult
stem cells from adipose tissue of multiple donors supports their potential utility
in future tissue-engineering applications.
Keywords
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References
- An established preadipose cell line and its differentiation in culture II. Factors affecting the adipose conversion.Cell. 1975; 5: 19-27
- Cellular and molecular aspects of adipose tissue development.Annu Rev Nutrition. 1992; 12: 207-233
- PPAR gamma: adipogenic regulator and thiazolidineone receptor.Diabetes. 1998; 47: 507-514
- Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium.J Clin Invest. 1989; 84: 1663-1670
- Differences in insulin action as a function of original anatomical site of nearly differentiated adipocytes obtained in primary culture.J Clin Invest. 1991; 88: 1629-1635
- Viability of fat obtained by syringe suction lipectomy: effects of local anesthesia with lidocaine.Aesthetic Plastic Surg. 1995; 19: 335-339
- Biochemical assessment of cellular damage after adipocyte harvest.J Surg Res. 1997; 70: 95-100
- Surface protein characterization of human adipose tissue-derived stromal cells.J Cell Physiol. 2001; 189: 54-63
- Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: biochemical, cellular and molecular analysis.Metabolism. 2001; 50: 407-413
- Adipogenic potential of human adipose derived stromal cells from multiple donors is heterogeneous.J Cell Biochem. 2001; 81: 312-319
- Multilineage cells from human adipose tissue: implications for cell-based therapies.Tissue Eng. 2001; 7: 211-228
- Human adipose tissue is a source of multipotent stem cells.Mol Biol Cell. 2002; 13: 4279-4295
- Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells.Tissue Eng. 2001; 7 (): 729-741
- Myogenic differentiation by human processed lipoaspirate cells.Plast Reconstr Surg. 2002; 109: 199-209
- Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo.Biochem Biophys Res Commun. 2002; 290: 763-769
- Neurogenic differ entiation of murine and human adipose-derived stromal cells.Biochem Biophys Res Commun. 2002; 294: 371-379
- Multilineage potential of adult human mesenchymal stem cells.Science. 1999; 284: 143-147
- Human pluripotent and progenitor cells display cell surface cluster differentiation markers CD 10, CD 13 CD 56, and MHC class-I.Proc Soc Exp Biol Med. 1999; 221: 63-71
- Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.Anat Rec. 2001; 264: 51-62
- Number of osteo-progenitor cells in human bone marrow markedly decreases after skeletal maturation.J Bone Mineral Metab. 1999; 17: 171-177
- Maintenance of osteoblastic and adipocytic differentiation potential with age and osteoporosis in human marrow stromal cell cultures.Calcif Tissue Int. 2002; 71: 36-44
- Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow.J Bone Mineral Res. 1999; 14: 1115-1122
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© 2004 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
